Folks,

If you were a PI or  a Post Doc, what would be the topic of your research proposal? I am looking for some creative thinking from you guys. To be specific, it should more of a cell biology, immunology and less of biochemistry or biophysics as nano. 

Thanks for sharing your ideas (in advance :))

How about mutation? My theory is that mutation didn't happen just out of randomness. Mutation occured due to certain cell/gene obtaining outside aide (e.g bacteria affecting the cell) at certain time/place.

Another theory, Efficacy of drugs or potential of infection per human genome sequence (how certain sequence are more immune to it than others).

The above post makes me sad by virtue of its sheer stupidity. Don't know where to start the deconstruction but I'll make an attempt.

Let's start with 'theory'. One might propose a Hypothesis; it takes experimental evidence and concomitant reproducibility to establish a theory. 

Mutation has bacteria as 'aide'? Ever heard of UV light? The host-pathogen interactions are already established phenomena. If you had referred to co-evolution of interacting proteins, I could have offered you benefit of doubt. And certain time/place? Last time I checked, everything happens at a certain time/place. Such is the nature of nature.

Genome sequence 'immune' to drugs or infectious agents? I could stab myself for this. Do you even know how small molecules or infectious agents work? 

Bottom line: Whereof one cannot speak, thereof one must remain silent

@author:

If this is out of curiosity as to what the fascinating topics in molecular biology are, then you might receive some input; otherwise a person who cannot even think of a proposal topic is not to be trusted for its execution. Let's leave it at that.

Sajha used to be different only a few years ago. Oh, how the times have changed!

Zombie4 .. Myan I liked ur response.. RESPECT

So we have the Nobel laureate here and we are suppose to bow down and everybody else should just shut up as their sheer stupidity wreaks havoc on the masses.

Dude calm down, will you? You sure seem to have more knowledge than me on the subject (believe me it is not my subject of study) but take it down a notch on your egotistical thrashing. I presented mere theories and trust me they didn’t come baseless. If you claim to have the know-it-all attitude (that you want to shut everybody else off), then go read about the theory of introduction of mitochondria to protozoic cells (how one particular cell would evolve, which I think leans more towards modern synthesis than mutation) . You wanna stab yourself to death about genome sequence? Be my guest, but wait till the news of a company involved in human genome sequence decoding at a rapid pace (minutes as oppose to weeks) comes out and their theory which decides how the gene structure/sequence could determine susceptibility to a drug or bacterial infection; guess what the company will be out in a year or two.

If we don't have discussion how would we help? Sajha used to be different? Don't give me that clichéd line, I have been here for 5 years and other than losing some respectable authors, I find it very similar..still.

Now go ahead come up with your rantings. With so much knowledge on the subject, we prefer that you advise Narayangarh suburb on this thesis subject rather than that Charlie-Sheen rants….WINNING.

I doubt if he got ur line zombie !!! He's still bragging 
"whereof one cannot speak, thereof one must remain silent" re k stiffu bro

Peace

Fat beast bro, did you even get what I am saying? How am I the one bragging now?
And also, that line applies to you as well.

Omg, are u guys meant to help this thread opener or argue yourselves? You guys behave like "hamro desh ko neta". Just chill and suggest this guy some topics. Who knows may be one day he might prove the "most stupid" thought posted in here!!

You know what am talking abt stiffu dai...u cant be good at everything, com'on myannn !!! If u already know that's not something of ur field of study then why don't UUU take it down a notch on your I-know-it-all bragging.. 

Love u stiffu Dai

eternalsunshine
This is what I call a civic conversation. I hope to be more enlightened on this subject which I have merely touched the surface of on my leisure time. Please keep them coming.

First please be aware of saying “my theory,” apparently we are expected to present experimental evidence to claim it a theory. Jokes aside, I see your point about DNA polymerase. What I am curious is why would these enzymes not play by the rule all the time and just catalyze the nucleotides into DNA given a template to copy from. My doubt is with the so called intelligence we assign them as in Darwin theories. You might counter that we don’t need to assign any intelligence here, but how else would it decide to mutate 1 in a million nucleotide it helps polymerize? Besides isn’t there a set restrictions on which strand it can add the nucleotides to etc etc? My theory (ahem..thought) is that environment kicks in and once in, say million, the timing and placement is just so right that the change (or mutation) occurs. There has been some new theories on how we developed limbs and what role did bacteria play in the early cell; even proposes mitochondria to have been introduced to the cell by environment. To align with that theory then, it would make more sense that randomness (or mutation) doesn’t happen, environmental changes force these mutation and the mutation that can survive will last. Why would one in a million population get hit by lightening? Because he was there at the right (or wrong) time/right place; rather than saying nature thought it was less of a survivor.

Would love to read more from you.
Fatbeast bhai, not trying to ignore you but I have nothing much to argue on something that doesn’t make sense. Jadhau and God bless your heart.

No wonder that didn't make any sense to u stiffu dai... Lato desh ko gado tannerri

 Haha,

I am loving it. chill out dudes. I can't beat the noble laureate ZOMBIE..afterall in this era everyone likes zombie.  However, my humble request to zombie, show me atleast one nature publications of yours and I am out of here, OR STFU. 

 ok here's the thing
mutation is random. you cannot induce a specific mutation at a site. if you could do that then BAM there a nobel prize for you. unfortunately you can only induce random mutations and select for the one that interests you.
secondly, i love the principle of darwin, how?-we are the progeny that have been selected for several thousand years. dont you love when someone says that. you are special. now the real question is how have we evolved. if you ask darwin he might have a different angle of seeing the same thing as todays molecular biologist see. the point is we are selected as a random event of mutations. when you were concieved, there were around 1 mils of your borthers and sisters who competed with you, but what made you so special that you won the race. i dont know the right answer but my guess is you must have inhereted a special mutation that enabled you to swim faster than your borthers and so you are here. the point is mutation is random and in that milion only 1 or may be few survive. this environment is a real killer. if you cant change to suite you have to die. thats the pressure of selection. you were selected.
if you have read immunology text book, there's context that can explain what is a selection pressure and how will a cell be selected or eliminated. its the reaction in the germinal center in which a B cell has to be selected. the out come of this process is that your body will produce more reactive antibody producing B cells. how does it do that? well my fren it a job of a cellular protein that induces random hypermutations. now when new progenies are produced, they will be selected. any one with has strong reactivity will be allowed to survive and one with less or no reactivity will be eliminated. the one which survived will reproduce in a clonal manner and produce more of its kind.
the idea is mutations are random but there a selecting force called mother nature. 
@stiffler, i dont know how have you emersed yourseld in science. but science works with "my theory". i wouldn't always believe in the existing data. 

 Now, although the discussion in this thread is going in a entirely different direction, I want to correct this line "when you were concieved, there were around 1 mils of your borthers and sisters who competed with you, but what made you so special that you won the race". As far as I recall from the course in reproduction physiology, only one sperm makes it to the ova. There are various projective enzymes and caspases! ( I am not sure of capsase cascade, but I believe, since it induces the apoptosis, It is there too). in the uterine environment. The head region of sperm has to go through capacitation, with influx of calcium and activation of cAMP (cyclic adenosine monophosphate), a signal transduction pathway and thus release of acrosomal enzymes. So, I believe one sperm one ova leads to fertilization as far as I recall. 

@Narayangarh, I believe you are right on the methodology (how about twins?) but eternalsunshine (of a sportless mind)'s point was about mutation on how one sperm might have been "mutated" to lead the way.

@eternal, the reference on "my theory" was merely a humble satire to the Nobel laurete we have in this thread, read his posting and you will understand. At this time, I still find it hard to believe in mutation Maybe one of the sperm had the right angle or tasted the right mix..whatever. Mutations in the past (or even now) are not induced purposefully, it just happened that the conditions were ripe (like presence of certain bacteria, other physical traits like temp/pressure in that region of the cell)to bring upon the mutation. But, since I have no experiments to prove my theory, I have to laydown my arguments as I definately need more understanding of the subject to counter what you have said. Thanks for knowledgable input/discussion. Help this Narayangarh friend of ours now.

https://www.youtube.com/watch?v=ySw0RVO3dwI

 @ stiffler sorry 

 Did George Carlin run into you fools when he said 'never underestimate the power of stupid people in large groups'? Two fools whose gaps in knowledge are bigger than Oprah's ass are serenading each other in blather. One has a misplaced understanding of endosymbiotic theory and the other hasn't heard of site-directed mutagenesis and yet proclaims mutation cannot be induced at a specific site! Community college too hard for you, eh? 

Two pieces of advice (one is a repetition) courtesy of Wittgenstein:

1) Whereof one cannot speak, thereof one must remain silent
 
2) Dont' try and shit higher than your arse

 @ author: Will 'Cell' do for ya? 


Keep on polluting Sajha. I'm done wasting my time with retards.